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Cinnamaldehyde, the main compound of cinnamon, has shown various effects in the liver; however, its cytotoxicity has not been fully investigated. In the present study, we examined the effect of cinnamaldehyde on adult rat hepatocytes. Adult male Wistar rats were fed with 30 mg/kg of cinnamaldehyde suspended in drinking water for up to 28 days. Rats were killed on day 14 and day 28. At each time point, five animals were sacrificed and their livers were removed, embedded in paraffin, and thin sectioned. Tissues were stained with hematoxylin and eosin to evaluate histologic abnormalities. Cleaved caspase-3 protein levels were measured by immunostaining with specific antibody. In addition, expression of growth arrest and DNA damage 45 alpha protein (GADD45A), growth arrest and DNA damage 45 beta protein (GADD45B), and GADD45gamma protein were evaluated by immun

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