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The neuropeptide, neuropeptide S (NPS) shares high homology with a group of atrial natriuretic peptides (ANP) and coordinates cardiovascular and gastro-intestinal systems during stress responses. To identify the physiological roles of NPS in feeding behavior, we examined the effect of systemic injections of NPS on food intake and on the release of the satiety hormones, pituitary adenylate cyclase-activating polypeptide (PACAP), corticotropin-releasing hormone (CRH) and peptide YY (PYY) from the gut. I.p. injection of NPS into rats was followed by a delayed increase in food intake. The feeding response induced by NPS was not accompanied by a change in the secretion of PYY from the gut nor by altered expression of PACAP and CRH in the brain. However, the two gastric adenylate cyclase-stimulating peptides, PACAP27 and PACAP38, were both slightly increased in NPS-treated rats. The NPS response was restricted to the CNS and appeared to be mediated at the hypothalamic level. Results suggest that NPS may act as a brain-derived satiety peptide and influences feeding behavior via the release of PACAP, a hypothalamic peptide, from the melanocortin system.Don,
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