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Anaphylactic shock is a life-threatening emergency caused by antigen stimulation in susceptible individuals. In allergic and anaphylactic reactions, mast cells (MCs) are involved in the development of allergy and anaphylaxis. MCs express numerous types of reactive oxygen species (ROS), including the superoxide anion radical, hydrogen peroxide, hydroxyl radicals, and singlet oxygen. The aim of this study was to determine whether procyanidin B2 (PB2), a flavonoid compound of cocoa, prevents the reactive oxygen species (ROS) production and the activation of MCs. PB2 was able to scavenge superoxide, hydrogen peroxide, hydroxyl, and singlet oxygen radicals, whereas it did not directly scavenge the hydrogen peroxide radical. Furthermore, PB2 was shown to inhibit the ROS production in a dose-dependent manner. In addition, PB2 suppressed the activation of MCs by inhibiting intracellular Ca2+ mobilization and leukotriene (LT) C4 production. Moreover, PB2 inhibited cyclooxygenase (COX)-2 protein expression and lipopolysaccharide (LPS)-induced inducible nitric oxide synthase (iNOS) expression in LPS-stimulated RAW264.7 macrophages. These findings indicate that PB2 suppresses ROS production and inflammatory mediator production in MCs. Therefore, PB2 is expected to be useful for the prevention of anaphylactic shock.Botulinum toxin (BTX) type A injections for therapy of functional benign idiopathic trigeminal neuralgia: a
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