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Ovariectomy of adult female rats alters the circadian rhythm of adenohypophysial CRH mRNA levels and CRH-releasing activity (CRA). Although under a 12:12 light-dark cycle these responses usually appear around the fourth post-ovariectomy day, day-night differences in CRH mRNA levels also appear at the seventh or eighth day after ovariectomy. These responses of CRH mRNA levels and CRA to ovariectomy depend on oestrogen input. Moreover, ovariectomy of adult female rats also alters the nocturnal rise in rectal temperature, which is not oestrogen dependent. We hypothesize that the nocturnal rise in temperature increases hypothalamic CRH mRNA levels, and that temperature is the primary regulator of the CRH mRNA response to ovariectomy. To test this hypothesis, we measured nocturnal changes in hypothalamic CRH mRNA levels in ovariectomized rats housed at either warm or cold ambient temperatures. We also measured nocturnal changes in hypothalamic and plasma CRH levels in ovariectomized rats housed at either warm or cold ambient temperatures. Following 8 days ovariectomy, the CRH mRNA response was suppressed in warm-acclimated rats, but there was no difference in CRH mRNA levels between warm-acclimated and cold-acclimated ovariectomized rats. We interpret these results to indicate that temperature, not ovariectomy, is the primary regulator of hypothalamic CRH mRNA levels in adult female rats. Finally, we also used a long-term (4-day) temperature-lowering regimen to demonstrate that the elevation in nocturnal rectal temperature supp
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Conclusions {#sec:conclusions}
===========
In this study, we have carried out an investigation into the impact of the beam centring effects on the velocity-Doppler velocimeter measurements of inclined magnetic field by the EFI method [@2016ApJ…830…94D]. We have demonstrated that it is possible to use Stokes $I$ signals to quantify the