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TIM-33-mediated regulation of the unfolded protein response in Neurospora crassa.
The unfolded protein response (UPR) is a cellular defense mechanism against the accumulation of unfolded or misfolded proteins in the endoplasmic reticulum (ER). Cells use the UPR to re-direct ER-associated protein folding toward protein degradation or to reduce protein synthesis. TIM-33 is a protein of unknown function with homology to the circadian regulators, CRY1 and CRY2. Here we show that expression of the TIM-33 transcript in Neurospora crassa is induced during ER stress. Deletion of the TIM-33 gene has a pleiotropic effect on the UPR: it results in reduced production of UPR-induced glycerol-
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