Akinsoft Cafeplus 9.02.07 Crack.14 ((NEW))

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Recovery of sialylated receptors in Rat Brain Striatal Neurons after Lys-M-Cre-Mediated Deletion of ST3Gal6.
Sialylation is an important cell surface glycosylation modification, which is involved in cellular functions. Previous studies found that sialylation of the alpha-amino group of N-acetylgalactosamine is required for an efficient process of some neuronal receptors, such as nerve growth factor receptor (TrkA) and delta opioid receptor. In this study, we investigated whether ST3Gal6, which regulates the alpha-2,3 and alpha-2,6 sialylation, affects the neuronal localization of sialylated receptors. For this purpose, we generated a Cre-transgenic mouse line with a Lys-M promoter and Cre recombinase coding sequence. The Lys-M-Cre mouse line with or without the Cre transgene (LysM- Cre, LysM- ), respectively, was used to express Cre recombinase in LysM- expressing cells, such as microglia, and remove the floxed gene for ST3Gal6 in the mouse brain. The ST3Gal6 was ablated from LysM- cells, but not LysM+ cells, in the brain. The ST3Gal6 expression level was lower in LysM- than LysM+ cells in the brain of the LysM- mouse line. In the cortex and striatum of the LysM-, but not LysM+ mice, the density of sialylated receptors such as TrkA and alpha-7 nicotinic acetylcholine receptor (alpha7nAchR) was significantly decreased. Likewise, the localization of these sialylated receptors was changed from punctate to diffuse in both LysM- and LysM+ cell groups. These results suggest that the ST

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