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Two and a half months ago, I attended the 2015 Radio Communications Conference. While I was at the conference, I had the opportunity to meet and speak with some key people in the industry.
When preparing for the conference, I decided to visit with a couple of folks, and spend some time talking about OANDA, who are a key player in the live performance market.
Over the last couple of days, I have had the pleasure of speaking with a number of people. While talking with them, it became clear that it would be a good idea to highlight the group I visited.
OANDA
Like many of the people I spoke with, I know OANDA well. I used them as a reference point for the conference, and as a key point for people wanting to know more about who we are and what we offer.
While I was at the conference, I met with Jared Moriarty, the Technical Marketing Manager for OANDA. Jared offered me the opportunity to show off some of the key technologies we use to deliver the world’s best live performance.
I have also received an offer to visit with Michael Rennie, the Sales Director for OANDA. I am looking forward to hearing more about the company.
Liquid Jazz
Like many of the other people I spoke with at the conference, Jason Tiberius from Liquid Jazz International was one of the individuals I wanted to meet. Having met with them, I have been given the opportunity to tell their story.
Liquid Jazz is an international, full service Broadcast Audio Company. Over the years, they have built an extensive business around delivering audio solutions to broadcasters and events.
I spoke with Jason for about an hour, and was able to listen to the entire range of services they have to offer.
Jason is one of the key people behind the New York City NY radio and sports broadcasts. As a full service company, they are there to provide audio for New York City – sports, radio, internet streams, news, corporate events, musical acts and more.
Jason provided me with a taste of their services, and was one of those on the conference call who wanted
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Dependent on the knockdown efficiency. For a successful knockdown, plasmid amounts >5 μg/well should be used.
Reference
Moffat, M., Khoo, C. S. H., and Ghosh, R. (2002). Morpholino antisense
oligonucleotides are powerful tools for gene function studies in fish.
Embo J. 21:1530-1535.
Knockdown affects the developmental stages of the embryos at late
stages. Embryos injected with morpholino antisense oligo are fertile. Embryo
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S2 cells were cultured as described in Methods. Cells were collected in
presence of 0.5% sodium dodecyl sulfate (SDS). Whole-cell lysate was
extracted in Laemmli sample buffer and subjected to polyacrylamide gel
electrophoresis followed by Western blot analysis. Endogenous laminin γ1 was
specifically detected with anti-laminin γ1.
Inhibition of laminin γ1 chain splicing was accomplished by the use of
a morpholino oligo complementary to a sequence in intron 2 (table 2) which is
detected by the MabA13 antibody. The morpholino oligo was designed to inhibit
splicing at the 5′-splice site (first arrow) and the branchpoint of intron 2
(second arrow). The blocking of these splicing events results in the retention
of the mRNA intermediate and an increase in the level of laminin γ1 mRNA.
Knockdown of laminin γ1 results in a reduction of the expression level of the
docked form of laminin γ1 and a concomitant increase in the expression level
of the undocked form of laminin γ1 which is detected by the MabA13 antibody.
This MabA13 antibody can be used for subsequent experiments on laminin
γ1 expression regulation. For example
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Carbon-based substrates have been used in thin-layer chromatography (TLC) and liquid chromatography (LC), also referred as capillary, for the separation of mixtures of organic compounds. A relatively time-consuming step remains the transfer of the carrier from the TLC/LC plates to the microplates for further analysis. In the present work, we have developed a simple and robust pretreatment protocol for the transfer of photopolymerized polycarbonate from chromatographic columns to analytical microplates. After photopolymerization and densification of the polycarbonate, a subsequent incubation in water allowed for the dissolution of the polycarbonate in water, achieving the contact with a layer of water on the analytical microplate. After evaporation of the remaining water, a thin layer of polycarbonate was left on the analytical microplate. The presence of the polycarbonate on the analytical microplate was confirmed by optical microscopy and direct methanol-substrate Raman analysis. The transfer methodology was validated for the transfer of a polycarbonate chromatographic column to analytical microplates. A wide range of analytical signals is expected when using this transfer protocol.
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